Kinetoplast DNA minicircles of phloem-restricted Phytomonas associated with wilt diseases of coconut and oil palms have a two-domain structure

dc.contributor.authorMichel Dollet
dc.contributor.authorNancy R. Sturm
dc.contributor.authorJean-Charles Ahomadegbe
dc.contributor.authorDavid A. Campbell
dc.date.accessioned2014-05-07T09:16:31Z
dc.date.available2014-05-07T09:16:31Z
dc.date.issued2001
dc.description.abstractWe report the cloning and sequencing of the first minicircle from a phloem-restricted, pathogenic Phytomonas sp. (Hart 1) isolated from a coconut palm with hartrot disease. The minicircle possessed a two domain structure of two conserved regions, each containing three conserved sequence blocks (CSB). Based on the sequence around CSB 3 from Hart I, PCR primers were designed to allow specific amplification of Phytomonas minicircles. This primer pair demonstrated specificity for at least six groups of plant trypanosomatids and did not amplify from insect trypanosomatids. The PCR results were consistent with a two-domain structure for other plant trypanosomatids.en_US
dc.identifier.citationFEMS Microbiology Letters 205 (2001) 65-69en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1035
dc.language.isoenen_US
dc.publisherElsevier Scienceen_US
dc.subjectConserved sequence blocken_US
dc.subjectHartroten_US
dc.subjectMarchitez sorpressivaen_US
dc.subjectPolymerase chain reactionen_US
dc.subjectPlant trypanosomatiden_US
dc.titleKinetoplast DNA minicircles of phloem-restricted Phytomonas associated with wilt diseases of coconut and oil palms have a two-domain structureen_US
dc.typeArticleen_US

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