Unfertilized Ovary: A Novel Explant for Coconut (cocos Nucifera L.) Somatic Embryogenesis

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dc.contributor.authorPrasanthi I.P. Perera
dc.contributor.authorValerie Hocher
dc.contributor.authorJean Luc Verdeil
dc.contributor.authorSylvie Doulbeau
dc.contributor.authorDeepthi M.D. Yakandawala
dc.contributor.authorKaushalya Weerakoon, L.
dc.date.accessioned2014-05-21T09:14:52Z
dc.date.available2014-05-21T09:14:52Z
dc.date.issued2007-01
dc.description.abstractUnfertilized ovaries isolated from immature female flowers of coconut (Cocos nucifera L.) were tested as a source of explants for callogenesis and somatic embryogenesis. The correct developmental stage of ovary explants and suitable in vitro culture conditions for consistent callus production were identified. The concentration of 2,4-dichlorophenoxyacetic acid (2,4-D) and activated charcoal was found to be critical for callogenesis. When cultured in a medium containing 100 µM 2,4-D and 0.1% activated charcoal, ovary explants gave rise to 41% callusing. Embryogenic calli were sub-cultured into somatic embryogenesis induction medium containing 5 µM abscisic acid, followed by plant regeneration medium (with 5 µM 6-benzylaminopurine). Many of the somatic embryos formed were complete with shoot and root poles and upon germination they gave rise to normal shoots. However, some abnormal developments were also observed. Flow cytometric analysis revealed that all the calli tested were diploid. Through histological studies, it was possible to study the sequence of the events that take place during somatic embryogenesis including orientation, polarization and elongation of the embryos.en_US
dc.identifier.citationPlant Cell Reports Vol.: 26, No.: 1, January 2007 [Page 21-28]en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1552
dc.language.isoenen_US
dc.publisherSpringer-Verlagen_US
dc.subjectsomatic embryogenesisen_US
dc.subjectcoconuten_US
dc.subjectovary cultureen_US
dc.subjecthistologyen_US
dc.subjectflow cytometryen_US
dc.titleUnfertilized Ovary: A Novel Explant for Coconut (cocos Nucifera L.) Somatic Embryogenesisen_US
dc.typeArticleen_US

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