ITS-RFLP characterization of black Aspergillus isolates responsible for ochratoxin A contamination in cocoa beans

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dc.contributor.authorFatima Bisbal
dc.contributor.authorJose Vicente Gil
dc.contributor.authorDaniel Ramon
dc.contributor.authorPedro Vicente Martinez-Culebras
dc.date.accessioned2014-04-11T06:35:21Z
dc.date.available2014-04-11T06:35:21Z
dc.date.issued2009
dc.description.abstractIn the present study, ochratoxigenic mycobiota in cocoa beans was identiWed at species level by digestion of the ITS products using the endonucleases HhaI, NlaIII and RsaI. Of the 132 isolates of Aspergillus section Nigri collected from cocoa beans, 89 were identiWed as A. tubingensis, 27 as A. niger, 10 as A. tubingensis-like and 6 as A. carbonarius. No variation was observed between RFLP patterns (C, N, T1 and T2) described previously for grape isolates and those of the cocoa isolates analysed. With respect to OTA-producing fungi, a high percentage of black aspergilli (50.7%) was able to produce OTA. Additionally, most of the OTA-producing isolates were of moderate toxigenicity, producing amounts of OTA from 10 g g¡1 to 100 g g¡1. Percentages of OTA-producing isolates in the A. niger aggregate were higher than in other substrates, ranging from 30% to 51.7%. Furthermore, the detected levels of OTA production in the A. niger aggregate, particularly in A. tubingensis species was higher than in A. carbonarius, ranging from 0.7 g g¡1 to 120 g g¡1 (mean 24.55 g g¡1). Due to the high occurrence, percentage of ocratoxigenic isolates and their ability to produce OTA, isolates belonging to the A. niger aggregate could be considered as the main cause of OTA contamination in cocoa beans used for manufacturing cocoa products.en_US
dc.identifier.citationEur Food Res Technol (2009) 229:751–755en_US
dc.identifier.urihttp://hdl.handle.net/123456789/366
dc.language.isoenen_US
dc.subjectBlack aspergillien_US
dc.subjectCocoa beansen_US
dc.subjectCocoa productsen_US
dc.subjectFood safetyen_US
dc.subjectIdentiWcationen_US
dc.subjectOchratoxin Aen_US
dc.subjectRFLP analysisen_US
dc.titleITS-RFLP characterization of black Aspergillus isolates responsible for ochratoxin A contamination in cocoa beansen_US
dc.typeArticleen_US

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