Isolation and characterization of polymorphic micro satellites in Cocos nucifera L.
| dc.contributor.author | Rivera, R. | |
| dc.contributor.author | Edwards, K.J. | |
| dc.contributor.author | Barker, J.H.A. | |
| dc.contributor.author | Arnold, G.M. | |
| dc.contributor.author | Ayad, G. | |
| dc.contributor.author | Hodgkin, T. | |
| dc.contributor.author | Karp, A. | |
| dc.date.accessioned | 2014-05-07T07:03:45Z | |
| dc.date.available | 2014-05-07T07:03:45Z | |
| dc.date.issued | 2007-02-08 | |
| dc.description.abstract | Micro satellites or simple sequence repeats (SSRs) were isolated from coconut (Cocos nucifera) and tested for polymorphism on restricted germplasm. Sequencing of 197 clones from a cv. Tagnanan Tall-enriched genomic library showed that 75% contained a microsatellite, of which 64% were dinucleotide (GA/CT, CA/GT and GC/CG), 6 were trinucleotide, and 30% were compound repeats. Of 41 primer pairs tested on Tagnanan Tall genomic DNA, 38 gave the expected size product, two amplified two loci, and another gave a multilocus pattern. On 20 coconut sample-the 38 SSRs detected 198 alleles (average: 5.2 alleles per microsatellite). Genetic diversity (D = I - 1 p,2) values ranged from 0.141 to 0.809. Heterozygotes were present at high frequencies among some dwarf samples. Analysis of similarity matrices based either on shared alleles at each locus (simple matching coefficient) or on allele bands across all loci (Jaccard coefficient) showed similar results. Dwarfs grouped separately from tails and showed less genetic diversity. In a wider test on 40 samples, 8 SSRs detected 64 alleles (average: eight alleles per microsatellite). These results indicate the high potential of microsatellites lo detect genetic diversity in coconut germplasm. | en_US |
| dc.identifier.citation | Genome 1999 v.42(4) p-668-675 | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/1023 | |
| dc.language.iso | en | en_US |
| dc.subject | molecular markers | en_US |
| dc.subject | microsatellite | en_US |
| dc.subject | SSR | en_US |
| dc.subject | Cocos nucifera | en_US |
| dc.subject | coconut | en_US |
| dc.title | Isolation and characterization of polymorphic micro satellites in Cocos nucifera L. | en_US |
| dc.type | Article | en_US |