Isolation of beta-mannanase from Cocos nucifera Linn haustorium and its application in the depolymerization of beta-(1 ,4)-linked D-mannans
Loading...
Date
2010-05
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Beta mannanase was extracted from coconut(Cocos nucifera Linn) haustorium and purified through ammonium sulphate precipitation and sepharose 6B-lectin affinity chromatography.Coconut beta mannanase is an acidic protein with a pI of 3.75.The molecular mass of coconut beta mannanase (sodium dodecyl sulfate-polyacrylamide gel electophoresis) was found to be 44kDa and was confirmed by matrix-assisted lase desorption ionization time-of-flight mass spectrometry.the optimum temperature and pH for enzyme activity was 70c and 5.2.the enzyme was used for the preparation of neutraceutical dietary supplement from glactomannans of guar gum and tender coconut kernel having a beta-(1,4)-linked D-mannanose backbone.Depolymerised guar gum has 92% of oligosaccharides with a degree of polymerization of 9-39 oligosaccharides along with disaccharides and trisaccharides. Hence this mannanase will be useful to depolymerize beta-(1,4)-linked D-mannose poly-saccharides from the most plant sources to produce prebiotics in a cost-effective technique.
Description
Keywords
Galactomannan, Beta mannanase, haustorium, Cocos nucifera Linn, prebiotic
Citation
International Journal of Food Sciences and Nutrition May 2010 Vol.61(3) p.272-281