SDS-Page of the High-Molecular-Weight Subunits of Wheat Glutenin and the Characterization of 1R (1B) Substitution and 1BL/1RS Translocation Lines

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Date

1984

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Abstract

The high-molecular-weight subunits of glutenin from wheat 1 R( 1B) substitution and 1BL/1RS translocation lines were fractionated by SDS-PAGE. Two new subunits denoted R[ and R2 were characterized in 1R(1B) substitution, but not in IBL/IRS translocation lines. Rt and R2 were proved to be rye proteins by 2d electrophoresis(NEPHGE x SDS-PAGE). In contrast to literature citations it was demonstrated that the cultivar Winnetou is a 1R(1B) substitution line and the cultivars Clement and Mildress both are 1BL/1RS translocation lines.

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Keywords

Triticum aestivum, wheat, glutenin, high molecular-weight subunits of glutenin, SDS-PAGE, 1R(1B) substitution identification, 1BL/1RS translocation identification, bread-making quality

Citation

Euphytica 33(1984) 3-8

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