Subunit structure of the vicilin-like globular storage protein of cocoa seeds and the origin of cocoa- and chocolate-specific aroma precursors
| dc.contributor.author | Ulrich Kratzer | |
| dc.contributor.author | Ronald Frank | |
| dc.contributor.author | Hubert Kalbacher | |
| dc.contributor.author | Bole Biehl | |
| dc.contributor.author | Johannes Wostemeyer | |
| dc.contributor.author | Jurgen Voigt | |
| dc.date.accessioned | 2014-04-17T11:13:52Z | |
| dc.date.available | 2014-04-17T11:13:52Z | |
| dc.date.issued | 2009 | |
| dc.description.abstract | Essential cocoa-specific aroma precursors are generated during fermentation of cocoa seeds by proteolysis of the vicilin-like globulin. This particular storage protein consists of three subunits with apparent molecular masses of 47 kDa, 31 kDa, and 15 kDa, respectively, which are derived from a common 66- kDa precursor. Since all these subunits resist N-terminal sequencing by Edman degradation, we have analysed the localisation of these vicilin subunits on their common precursor sequence by MALDI-TOF-MS analyses of their tryptic fragments. The results were corroborated by epitope mapping of polyclonal antibodies using 185 overlapping pentadeca-peptides covering the whole sequence of the precursor. The results show that the cocoa vicilin is encoded by a single gene and that heterogeneity of the vicilin subunits must be attributed to statistical post-translational modifications. Localisation of the precursor regions, from which the various subunits are derived, will be helpful for the future identification of aroma-relevant peptides generated during the fermentation process. | en_US |
| dc.identifier.citation | Food Chemistry 113 (2009) 903–913 | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/427 | |
| dc.language.iso | en | en_US |
| dc.subject | Cocoa seeds | en_US |
| dc.subject | Epitope mapping | en_US |
| dc.subject | MALDI-ReTOF-MS | en_US |
| dc.subject | Storage protein | en_US |
| dc.subject | Theobroma cacao | en_US |
| dc.subject | Vicilin | en_US |
| dc.title | Subunit structure of the vicilin-like globular storage protein of cocoa seeds and the origin of cocoa- and chocolate-specific aroma precursors | en_US |
| dc.type | Article | en_US |