Genetic variation in somatic embryogenic response in open-pollinated families of black spruce
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Date
1991
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Abstract
Zygotic embryos from open-polIinated seeds of 20 black spruce (Pice a mariana) families were used to investigate the proportion of genotypes that would give rise to embryogenic tissue (ET) and mature somatic embryos. Eighty five percent of the maternal genotypes gave rise to embryogenic tissue Within-family rates of ET induction ranged from 0 to 17%, with an average of 8%. The largest proportion of variation was among families, indicating the additive nature of the genetic variation. On a medium with 6% sucrose and 3.7 microM ABA, 90% of the embryogenic lines gave rise to abundant
(> 100/100 mg of ET), well-formed, mature somatic embryos. A medium with 2% sucrose, without 2,4-D, was used to germinate the mature somatic embryos. These
were grown in the greenhouse and have now been estabfished in field trials.
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Keywords
Somatic embryogenesis, Picea mariana, Biotechnology
Citation
Theor Appl Genet (1991) 82:185-190