Use of SSR Markers to Determine the anther-derived Homozygous Lines in Coconut

Simple item page
dc.contributor.authorPerera, P.I.P.
dc.contributor.authorPerera, L.
dc.contributor.authorHocher, V.
dc.contributor.authorVerdeil, J.L.
dc.contributor.authorYakandawala, D.M.D.
dc.contributor.authorWeerakoon, L.K.
dc.date.accessioned2014-05-21T10:31:50Z
dc.date.available2014-05-21T10:31:50Z
dc.date.issued2008-01
dc.description.abstractAnther culture was used to obtain dihaploid (DH) coconut plants and their ploidy level was determined by flow cytometric analysis. Simple sequence repeat (SSR) marker analysis was conducted to identify the homozygous diploid individuals. Ploidy analysis showed that 50% of the tested plantlets were haploid and 50% were diploid. Polymorphic fragments of the mother palm and their segregation patterns in anther-derived plantlets were used to determine the origin of the diploid plantlets. Using a diagnostic SSR marker (CNZ43), all the diploid plantlets tested were identified as being derived from microspores (i.e. were homozygous) and were thus candidates for use in coconut breeding programs.en_US
dc.identifier.citationPlant Cell Reports Vol.: 27, No.: 11, January 2008 [Page 1697-1703]en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1566
dc.language.isoenen_US
dc.publisherSpringer-Verlagen_US
dc.subjectCoconuten_US
dc.subjectAndrogenesisen_US
dc.subjectDihaploiden_US
dc.subjectFlow cytometryen_US
dc.subjectSSR markeren_US
dc.titleUse of SSR Markers to Determine the anther-derived Homozygous Lines in Coconuten_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
use of ssr markers to determine the anther derived homozygous.pdf
Size:
359.22 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Coconut