An efficient method of DNA extraction from coconut leaves
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Date
1999
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Abstract
A protocol for extraction of good quality, high molecular weight genomic DNA from young leaves of coconut was standardized using two detergents viz- sodium dodecyl sulphate (SDS) and hexadecyl trimethyl ammonium bromide (CTAB) at different concentrations; at pH 8.0 and pH 9.0. SDS (1%) at pH 8.0 was found to yield good quantity of high quality DNA. The extracted DNA was amenable to PCR and restriction digestion
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Keywords
coconut, DNA, protocol, PCR, RFLP
Citation
Agrotropica 11(1): 35-38, 1999