Detection and Molecular Characterization of Phytoplasma associated with Lethal Yellowing Disease of Coconut Palms in Cuba

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dc.contributor.authorLlauger, R.
dc.contributor.authorBecker, D.
dc.contributor.authorCueto, J.
dc.contributor.authorPeralta, E.
dc.contributor.authorGonzalez, V.
dc.contributor.authorRodriguez, M.
dc.contributor.authorRohde, W.
dc.date.accessioned2014-04-25T10:35:56Z
dc.date.available2014-04-25T10:35:56Z
dc.date.issued2002
dc.description.abstractLethal Yellowing (LY) disease of coconut palm (Cocos nucifera L.) in Cuba has been reported since the end of the 19th century. In order to ascertain the presence of phytoplasmas associated with this disease, leaf samples were taken from plants showing typical disease symptoms and assayed for the LY agent by the polymerase chain reaction (PCR) using LY-specific primers. Selected PCR amplification products were cloned, sequenced and compared to that of a Mexican LY isolate from the Yucata´n region. The results obtained confirm the presence of LY phytoplasma in Cuba. Cuban and Mexican isolates show an overall high degree of sequence similarity with occasional point mutations and small deletions or insertions. Based on these identified genetic differences, LY isolates from the Havana and the Yucata´n region cluster together and apart from isolates originating at Maisı´ in eastern Cuba.en_US
dc.identifier.citationJ. Phytopathology 150, 390–395 (2002)en_US
dc.identifier.urihttp://hdl.handle.net/123456789/579
dc.language.isoenen_US
dc.subjectDNA cloningen_US
dc.subjectgenetic diversityen_US
dc.subjectMollicutesen_US
dc.subjectPCR diagnosisen_US
dc.subjectsequence alignmenten_US
dc.titleDetection and Molecular Characterization of Phytoplasma associated with Lethal Yellowing Disease of Coconut Palms in Cubaen_US
dc.typeArticleen_US

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