Investigations of different strategies for high frequency regeneration of Dendrobium malones ‘Victory’

Simple item page
dc.contributor.authorSaima Anjum
dc.contributor.authorMuhammad Zia
dc.contributor.authorFayyaz Chaudhary, M.
dc.date.accessioned2014-05-07T05:55:42Z
dc.date.available2014-05-07T05:55:42Z
dc.date.issued2007
dc.description.abstractRegeneration of orchid, Dendrobium malones “Victory”, has been established by direct differentiation and through callus formation. With direct thin leaf section differentiation method, the number of protocorm like bodies (PLBs) formed from Thin Section (TS) explants obtained from a single leaf was 10 times more than that from a single whole leaf, in a short time period. This therefore suggested that the proliferation potential is distributed all along the seedling leaves and morphogenically competent cells were not limited in the basal ends or the tips of the leaves in TS of orchid leaf cultures. Full MS media supplemented with auxins/cytokinins either in single or in combination along with peptone, yeast extract, casein hydrolysate (each at 100 mg/l), banana powder (40 g/l), 15% coconut water and 2% sucrose have shown excellent results for high frequency regeneration in orchid cultures. In many higher plants, regenerating leaves responded by developing proliferative loci in some “Predetermined Regenerative” cells in the dermal layers by tissue culture methods.en_US
dc.identifier.citationAfrican Journal of Biotechnology Vol. 5 (19), pp. 1738-1743, 2 October 2006en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1007
dc.language.isoenen_US
dc.publisherAcademic Journalsen_US
dc.subjectDendrobium malonesen_US
dc.subjectprotocormen_US
dc.subjectcallusen_US
dc.subjectorganogenesisen_US
dc.titleInvestigations of different strategies for high frequency regeneration of Dendrobium malones ‘Victory’en_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
investigations of different strategies for high frequency.pdf
Size:
137.59 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Coconut