Cryopreservation of Coconut (Cocos Nucifera L.) Zygotic Embryos by Vitrification
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Date
2011
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Abstract
The present study investigates the effect of preculture conditions, vitrification and
unloading solutions on survival and regeneration of coconut zygotic embryos after
cryopreservation. Among the seven plant vitrification solutions tested, PVS3 was found to be the most effective for regeneration of cryopreserved embryos. The optimal protocol involved preculture of embryos for 3 days on medium with 0.6 M sucrose, PVS3 treatment for 16 h, rapid cooling and rewarming and unloading in 1.2 M sucrose liquid medium for 1.5 h. Under these conditions, 70-80% survival (corresponding to size enlargement and weight gain) was observed with cryopreserved embryos and 20-25% of the plants regenerated (showing normal shoot and root growth) from cryopreserved embryos were established in pots.
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Keywords
Cocos nucifera L., coconut, zygotic embryo, preculture, vitrification, PVS3, unloading
Citation
CryoLetters 32 (4), 317-328 (2011)