Cryopreservation of Coconut (Cocos Nucifera L.) Zygotic Embryos by Vitrification

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dc.contributor.authorSajini, K.K.
dc.contributor.authorAnitha Karun
dc.contributor.authorAmarnath, C.H.
dc.contributor.authorFlorent Engelmann
dc.date.accessioned2015-01-06T05:18:31Z
dc.date.available2015-01-06T05:18:31Z
dc.date.issued2011
dc.description.abstractThe present study investigates the effect of preculture conditions, vitrification and unloading solutions on survival and regeneration of coconut zygotic embryos after cryopreservation. Among the seven plant vitrification solutions tested, PVS3 was found to be the most effective for regeneration of cryopreserved embryos. The optimal protocol involved preculture of embryos for 3 days on medium with 0.6 M sucrose, PVS3 treatment for 16 h, rapid cooling and rewarming and unloading in 1.2 M sucrose liquid medium for 1.5 h. Under these conditions, 70-80% survival (corresponding to size enlargement and weight gain) was observed with cryopreserved embryos and 20-25% of the plants regenerated (showing normal shoot and root growth) from cryopreserved embryos were established in pots.en_US
dc.identifier.citationCryoLetters 32 (4), 317-328 (2011)en_US
dc.identifier.urihttp://hdl.handle.net/123456789/6110
dc.language.isoenen_US
dc.subjectCocos nucifera L.en_US
dc.subjectcoconuten_US
dc.subjectzygotic embryoen_US
dc.subjectprecultureen_US
dc.subjectvitrificationen_US
dc.subjectPVS3en_US
dc.subjectunloadingen_US
dc.titleCryopreservation of Coconut (Cocos Nucifera L.) Zygotic Embryos by Vitrificationen_US
dc.typeArticleen_US

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