A Quantitative Method For Extracting Resting Spores Of Two Nematode Parasitic Fungi, Nematophthora Gynophila And Verticillium Chlamydosporium, From Soil
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1981
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A method is described for quantitative extraction of Nematophthora gynophila and Verticillium chlamydosporium spores from soil. These two species of fungi can prevent populations of the cereal cyst-nematode, Heterodera avenae, increasing when susceptible cereal hosts "are grown. The soil sample is wet sieved and the spores separated by centrifugation on a high specific gravity solution of magnesium sulphate. The spore suspension is counted directly under a compound microscope (125 x ). About 80% of spores added to three soils of different texture were recovered. In a survey of 23 soils, spore numbers of both fungi were large where H. avenae failed to multiply on susceptible hosts, and small where the nematode was increasing or causing damage.
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Nematologica 27(1981): 330-339