Failure of Putative Nitrogen-fixing Bacteria to Fix Nitrogen
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1969
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Analyses, tests with isotopic nitrogen and tests for acetylene and isocyanide reduction, using both continuous and batch cultures, were made with seven strains of putative nitrogen-fixing bacteria and three local isolates. Only two (single strains of Mycobacterium flavum and Pseudomonas azotogensis) fixed nitrogen; the active pseudomonad differed in several respects from the organism originally reported. Other Pseudomonas, Nocardia and Azotomonas species and the three local isolates did not fix: some simulated nitrogen fixation in cultural tests most impressively, but proved simply to be very efficient scavengers of traces of fixed nitrogen.
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J. Gen. Microbiol. (1969), 58, 277-285