Chiral separation of (+)/(−)-catechin from sulfated and glucuronidated metabolites in human plasma after cocoa consumption
| dc.contributor.author | Christina Ritter | |
| dc.contributor.author | Benno F. Zimmermann | |
| dc.contributor.author | Rudolf Galensa | |
| dc.date.accessioned | 2014-04-02T04:40:52Z | |
| dc.date.available | 2014-04-02T04:40:52Z | |
| dc.date.issued | 2010 | |
| dc.description.abstract | Cocoa is well-known to be rich in flavan-3-ols. Previous analyses have established that alkaline treatment of cocoa beans results in epimerization of (−)-epicatechin to (−)-catechin and (+)-catechin to (+)-epicatechin. Now, the question is whether both epimers can be absorbed by the human organism. This paper describes sample preparation and an HPLC method for chiral determination of (+)/(−)- catechin from sulfated and glucuronidated metabolites in human plasma. The sample preparation includes enzymatic hydrolysis of the catechin metabolites, and solid-phase extraction (SPE). A PM-γ-cyclodextrin column is used with a coulometric electrode-array detection (CEAD) system. The recovery of catechin ranges from 89.9 to 96.8%. The limit of detection is 5.9 ng mL−1 for (−)-catechin and 6.8 ng mL−1 for (+)-catechin, and the limit of quantification is 12.8 ng mL−1 for (−)-catechin and 16.9 ng mL−1 for (+)- catechin. The relative standard deviation of the method ranges from 0.9 to 1.5%. This method was successfully applied to human plasma after consumption of a cocoa drink. In one human self-experiment, (+)-catechin and (−)- catechin were found in human plasma, but metabolism of the two enantiomers differed. | en_US |
| dc.identifier.citation | Anal Bioanal Chem (2010) 397:723–730 | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/164 | |
| dc.language.iso | en | en_US |
| dc.subject | Flavanols | en_US |
| dc.subject | Cocoa | en_US |
| dc.subject | Chiral | en_US |
| dc.subject | Human plasma | en_US |
| dc.subject | Electrochemical detection | en_US |
| dc.subject | HPLC | en_US |
| dc.title | Chiral separation of (+)/(−)-catechin from sulfated and glucuronidated metabolites in human plasma after cocoa consumption | en_US |
| dc.type | Article | en_US |