Allelic size standards and reference genotypes to unify international cocoa (Theobroma cacao L.) microsatellite data

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dc.contributor.authorCryer, N.C.
dc.contributor.authorFenn, M.G.E.
dc.contributor.authorTurnbull, C.J.
dc.contributor.authorWilkinson, M.J.
dc.date.accessioned2014-04-04T05:13:16Z
dc.date.available2014-04-04T05:13:16Z
dc.date.issued2006
dc.description.abstractStandardisation of microsatellite allele profiles between laboratories is of fundamental importance to the transferability of genetic fingerprint data and the identification of clonal individuals held at multiple sites. Here we describe two methods of standardisation applied to the microsatellite fingerprinting of 429 Theobroma cacao L. trees representing 345 accessions held in the worlds largest Cocoa Intermediate Quarantine facility: the use of a partial allelic ladder through the production of 46 cloned and sequenced allelic standards (AJ748464 to AJ48509), and the use of standard genotypes selected to display a diverse allelic range. Until now a lack of accurate and transferable identification information has impeded efforts to genetically improve the cocoa crop. To address this need, a global initiative to fingerprint all international cocoa germplasm collections using a common set of 15 microsatellite markers is in progress. Data reported here have been deposited with the International Cocoa Germplasm Database and form the basis of a searchable resource for clonal identification. To our knowledge, this is the first quarantine facility to be completely genotyped using microsatellite markers for the purpose of quality control and clonal identification. Implications of the results for retrospective tracking of labelling errors are briefly explored.en_US
dc.identifier.citationGenetic Resources and Crop Evolution (2006) 53:1643–1652en_US
dc.identifier.urihttp://hdl.handle.net/123456789/205
dc.language.isoenen_US
dc.subjectGermplasmen_US
dc.subjectMicrosatelliteen_US
dc.subjectQuarantineen_US
dc.subjectSSRen_US
dc.subjectTheobroma cacaoen_US
dc.titleAllelic size standards and reference genotypes to unify international cocoa (Theobroma cacao L.) microsatellite dataen_US
dc.typeArticleen_US

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