Neuroprotective effect of cocoa flavonids on in vitro oxidative stress
| dc.contributor.author | Emma Ramiro-Puig | |
| dc.contributor.author | Gemma Casadesus | |
| dc.contributor.author | Hyoung-gon Lee | |
| dc.contributor.author | Xiongwei Zhu | |
| dc.contributor.author | Andrew McShea | |
| dc.contributor.author | George Perry | |
| dc.contributor.author | Francisco J. Perez-Cano | |
| dc.contributor.author | Mark A. Smith | |
| dc.contributor.author | Margarida Castell | |
| dc.date.accessioned | 2014-04-16T06:23:45Z | |
| dc.date.available | 2014-04-16T06:23:45Z | |
| dc.date.issued | 2009 | |
| dc.description.abstract | Background Cocoa is a rich source of flavonoids that, among other functions, can act as antioxidants. In living systems, the production of reactive oxygen species (ROS) activate an array of intracellular cascades, including mitogen-activated protein kinases (MAPK), that are closely associated with cell death or survival pathways. Aim of the study To ascertain the role of a cocoa extract and its main flavonoid, (-)-epicatechin, in an in vitro model of oxidative stress induced in a neuronal cell line. Methods We analyzed ROS production by fluorometry (dichlorofluorescein assay), and activation of MAPK pathways including extracellular signal-regulated kinases 1/2 (ERK 1/2), c-Jun N-terminal kinase (JNK), and p-38, by Western blot analysis. Results Cells incubated with cocoa extract or (-)-epicatechin, reduced ROS production in a dose-dependent manner, reaching 35% inhibition. pJNK and p38, involved in apoptosis, were down-modulated by cocoa extract and (-)-epicatechin with p38 inhibition reaching up to 70%. Conclusions Our results show that cocoa extract and (-)-epicatechin may exert a neuroprotective action by reducing ROS production and modulating MAPK activation | en_US |
| dc.identifier.citation | Eur J Nutr (2009) 48:54–61 | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/393 | |
| dc.language.iso | en | en_US |
| dc.subject | cocoa | en_US |
| dc.subject | c-JNK | en_US |
| dc.subject | MAPK | en_US |
| dc.subject | neuronal damage | en_US |
| dc.subject | oxidative stress | en_US |
| dc.title | Neuroprotective effect of cocoa flavonids on in vitro oxidative stress | en_US |
| dc.type | Article | en_US |