In vitro organogenesis and plant formation in cucumber

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dc.contributor.authorSelvaraj, N.
dc.contributor.authorVasudevan, A.
dc.contributor.authorManickavasagam, M.
dc.contributor.authorGanapathi, A.
dc.date.accessioned2014-05-07T06:49:53Z
dc.date.available2014-05-07T06:49:53Z
dc.date.issued2006-03
dc.description.abstractIn vitro organogenesis was achieved from callus derived from hypocotyl explants of Cucumis sativus L. cv. Poinsett 76. Calli were induced from hypocotyl explants excised from 7-d-old seedlings grown on Murashige and Skoog (MS) medium containing 87.64 µM sucrose, 0.8 % agar, 3.62 µM 2,4-dichlorophenoxy acetic acid and 2.22 µM 6-benzyladenine (BA). Regeneration of adventitious buds from callus (25 shoots explant-1) was achieved on MS medium supplemented with 8.88 µM BA, 2.5 µM zeatin and 10 % coconut water after two subcultures in the same medium at 30-d interval. Gibberellic acid (1.75 µM) favoured shoot elongation and indole 3-butyric acid (7.36 µM) induced rooting. Rooted plants were hardened and successfully established in soil.en_US
dc.identifier.citationBiologia Plantarum Vol.: 50, No.: 1, March 2006 [Page 123-126]en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1018
dc.language.isoenen_US
dc.publisherSpringer-Verlagen_US
dc.subjectadventitious buden_US
dc.subjectcallusen_US
dc.subjectCucumis sativus L.en_US
dc.subjecthypocotylen_US
dc.titleIn vitro organogenesis and plant formation in cucumberen_US
dc.typeArticleen_US

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