Tissue Culture and Plant Regeneration of the Salt Marsh Monocots Juncus Roemerianus and Juncus Gerardi

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dc.contributor.authorDenise M. Seliskar
dc.contributor.authorJiangbo Wang
dc.contributor.authorJohn L. Gallagher
dc.date.accessioned2014-05-21T07:19:06Z
dc.date.available2014-05-21T07:19:06Z
dc.date.issued2005-05
dc.description.abstractPlant tissue culture media, sterilized through commercial filter membrane units, inhibited plant cell growth and development. Embryogenesis was suppressed in microspore cultures of Brassica napus and anther cultures of Triticum aestivum, and in vitro pollen development was suppressed in B. napus. Inhibition of growth and development occurred on media that had been filter-sterilized in low volumes through non-washed filter membrane units of all brands tested, including Nalgene, Corning, Millipore, and Sartorius membrane products. Similar results were obtained with Costar Transwells and Millipore inserts (Millicell-CM, -HA, and -PCF). These deleterious effects were eliminated by washing the membranes with water followed by a small volume of the medium. Media sterilized by filtration through washed filters produced significant improvements of growth and development of embryos and pollen.en_US
dc.identifier.citationIn Vitro Cellular and Developmental Biology: Plant Vol.: 41, No.: 3, May, 1 2005 [Page 281-284]en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1537
dc.language.isoenen_US
dc.publisherSpringer-Verlagen_US
dc.subjectblack needle rushen_US
dc.subjectblackgrassen_US
dc.subjectorganogenesisen_US
dc.subjectrestorationen_US
dc.subjectsalt marshen_US
dc.titleTissue Culture and Plant Regeneration of the Salt Marsh Monocots Juncus Roemerianus and Juncus Gerardien_US
dc.typeArticleen_US

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