Confirmation of nitrogen fixation in two tropical grasses by 15N2 incorporation
| dc.contributor.author | Helevecio De-Polli | |
| dc.contributor.author | Elichi Matsui | |
| dc.contributor.author | Johanna Dobereiner | |
| dc.contributor.author | Eneas Salati | |
| dc.date.accessioned | 2014-05-24T10:27:20Z | |
| dc.date.available | 2014-05-24T10:27:20Z | |
| dc.date.issued | 1977 | |
| dc.description.abstract | Intact soil cores containing plants of Paspalum notation or Digitaria decumbens were selected with the acetylene reduction method, and then exposed to 15N2 to confirm nitrogen fixation in tropical grass-bacteria associations. In a preliminary experiment with P. notatum 15N2 incorporation was slow but progressive during 24 h in roots but translocation to rhizomes and leaves ceased after 17 h. With improved assay chambers, enrichments of 0.151 and 0.563 15N atom % excess were obtained in roots of D. decumbens cv transvala and P. notatum systems respectively, after 3 days. Enrichments in rhizomes were similar to those of roots; however in the leaves only 8% of root enrichment was observed. The addition of sucrose to the soil doubled N2-fixation in roots in both grass species studied, but did not result in increased incorporation into the leaves of P. notatum. | en_US |
| dc.identifier.citation | Sol. Biol. Biochem., Vol 9, pp. 119-123 | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/1753 | |
| dc.language.iso | en | en_US |
| dc.title | Confirmation of nitrogen fixation in two tropical grasses by 15N2 incorporation | en_US |
| dc.type | Article | en_US |