Use of SSR markers to determine the anther-derived homozygous lines in coconut

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dc.contributor.authorPerera, P.I.P.
dc.contributor.authorVerdeil, J.L.
dc.contributor.authorHocher, V.
dc.contributor.authorPerera, L.
dc.contributor.authorYakandawala, D.M.D.
dc.contributor.authorWeerakoon, L.K.
dc.date.accessioned2014-04-25T05:26:25Z
dc.date.available2014-04-25T05:26:25Z
dc.date.issued2008
dc.description.abstractAnther culture was used to obtain dihaploid (DH) coconut plants and their ploidy level was determined by flow cytometric analysis. Simple sequence repeat (SSR) marker analysis was conducted to identify the homozygous diploid individuals. Ploidy analysis showed that 50% of the tested plantlets were haploid and 50% were diploid. Polymorphic fragments of the mother palm and their segregation patterns in anther-derived plantlets were used to determine the origin of the diploid plantlets. Using a diagnostic SSR marker (CNZ43), all the diploid plantlets tested were identified as being derived from microspores (i.e. were homozygous) and were thus candidates for use in coconut breeding programsen_US
dc.identifier.citationPlant Cell Rep (2008) 27:1697–1703en_US
dc.identifier.urihttp://hdl.handle.net/123456789/541
dc.language.isoenen_US
dc.subjectcoconuten_US
dc.subjectAndrogenesisen_US
dc.subjectDihaploiden_US
dc.subjectFlow cytometryen_US
dc.subjectSSR markeren_US
dc.titleUse of SSR markers to determine the anther-derived homozygous lines in coconuten_US
dc.typeArticleen_US

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