Diversity and phylogenetic profiling of niche-specific Bacilli from extreme environments of India

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dc.contributor.authorAjar Nath Yadav
dc.contributor.authorPriyanka Verma
dc.contributor.authorMurugan Kumar
dc.contributor.authorKamal K. Pal
dc.contributor.authorRinku Dey
dc.contributor.authorAlka Gupta
dc.contributor.authorJasdeep Chatrath Padaria
dc.contributor.authorGovind T. Gujar
dc.contributor.authorSudheer Kumar
dc.contributor.authorArchna Suman
dc.contributor.authorRadha Prasanna
dc.contributor.authorAnil K. Saxena
dc.date.accessioned2015-08-03T09:14:39Z
dc.date.available2015-08-03T09:14:39Z
dc.date.issued2015
dc.description.abstractThe diversity of culturable, aerobic and heterotrophic Bacillus and Bacillus-derived genera (BBDG) was investigated in various extreme environments (including thermal springs, cold deserts, mangroves, salt lakes, arid regions, salt pans and acidic soils) of India. Heat treatment followed by enrichment in different media led to a total of 893 bacterial isolates. Amplified ribosomal DNA restriction analysis (ARDRA) using three restriction enzymes AluI, MspI and HaeIII led to the clustering of these isolates into 12–74 groups for the different sites at 75 % similarity index, adding up to 559 groups. Phylogenetic analysis based on 16S rRNA gene sequencing led to the identification of 392 bacilli, grouped in two families, Bacillaceae (89.03 %) and Paenibacillaceae (10.97 %), and included 13 different genera with 75 distinct species. It was found that among the thirteen genera, nine (Bacillus, Halobacillus, Lysinibacillus, Oceanobacillus, Pontibacillus, Salinibaci l lus, Sediminibacillus, Thalassobacillus and Virgibacillus) belonged to Bacillaceae and four (Ammoniphilus, Aneurinibacillus, Brevibacillus and Paenibacillus) belonged to Paenibacillaceae. Novel isolates tolerant to low and high pH and temperature, salt and low moisture were identified. The major outcome of the present investigation was the identification of niche-specific species and also the ubiquitous presence of selected species of BBDG, which illustrate the diversity and pervasive nature of BBDG in extreme environments.en_US
dc.identifier.citationAnn Microbiol (2015) 65:611–629en_US
dc.identifier.urihttp://hdl.handle.net/123456789/6347
dc.language.isoenen_US
dc.subjectARDRAen_US
dc.subjectBacillusen_US
dc.subjectBacillus derived generaen_US
dc.subject16S rRNAgeneen_US
dc.subjectExtreme habitatsen_US
dc.titleDiversity and phylogenetic profiling of niche-specific Bacilli from extreme environments of Indiaen_US
dc.typeArticleen_US

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