Effect of Different Hormonal Combinations on Regeneration of Callus of Gomphrena globosa L.

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dc.contributor.authorPram Ghaffar
dc.contributor.authorBasharat Ali
dc.contributor.authorShahida Hasnain
dc.date.accessioned2014-04-26T10:26:22Z
dc.date.available2014-04-26T10:26:22Z
dc.date.issued2007
dc.description.abstractThe main aim of this study was to observe the effect of different hormonal combinations on regeneration of callus of Gomphrena globosa L. For this purpose callus was obtained from seeds G. globosa inoculated on MS medium supplemented with 4 mg L"1 2, 4-D and 10% coconut milk. After callus formation callus was inoculated on Murashige and Skoog's medium supplemented with different combinations of BAP, NAA and GA3 to observe different responses such as regeneration, callus friability, callus proliferation and pigmentation. In BAP and NAA root regeneration was observed at 1.5 mg L"1 BAP+1 mg L"1 NAA whereas rest of the combinations showed callus proliferation. In BAP and GA3, root regeneration was observed in most of the combinations and some combinations also showed shoot induction. Shoot regeneration was observed on 0.5 mg L~' BAP + 7 mg L~' GA3 and 1 mg L~' BAP + 0.2 mg L"1 GA3. The effect of all these combinations on auxin, acid phosphatase and soluble protein content was also observed.en_US
dc.identifier.citationPakistan Journal of Biological Sciences 10 (20): 3708-3712, 2007en_US
dc.identifier.urihttp://hdl.handle.net/123456789/649
dc.language.isoenen_US
dc.subjectGomphrenaen_US
dc.subjectcoconut milken_US
dc.subjectcallus proliferationen_US
dc.subjectpigmentationen_US
dc.subjectroot inductionen_US
dc.subjectauxinen_US
dc.titleEffect of Different Hormonal Combinations on Regeneration of Callus of Gomphrena globosa L.en_US
dc.typeArticleen_US

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