Storage and transport of zygotic embryos of Cocos nucifera L for in vitro culture

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dc.contributor.authorErlinda P. Rillo
dc.contributor.authorBelen F. Paloma, M.
dc.date.accessioned2014-05-19T10:24:40Z
dc.date.available2014-05-19T10:24:40Z
dc.date.issued2007-02-08
dc.description.abstractEmbryo culture of coconuts can circumvent problems of storage and transport caused by the weight and bulk of the nuts and the absence of dormancy. This paper describes a technique that can be routinely used for collecting large numbers of embryos. The embryos were extracted in the field, brought to a simple isolation room, washed in ethyl alcohol, disinfected in 1OO% commercial bleach for 20 minutes and then rinsed again. Sterilized embryos were sealed in sterile plastic bags and then transported in cold storage. Redisinfection in 100% commercial bleach for 5 minutes prior to culture under aseptic conditions was necessary to reduce contamination rates. This procedure did not adversely affect embryo germination.en_US
dc.identifier.citationPlant Genetic Resources News Letter v.86 p-1-4en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1475
dc.language.isoenen_US
dc.subjectcoconut
dc.subjectzygotic embryo-storage
dc.subjectzygotic embryo-transport
dc.subjectin vitro culture
dc.subject
dc.titleStorage and transport of zygotic embryos of Cocos nucifera L for in vitro cultureen_US
dc.typeArticleen_US

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