Maintenance of embryogenic potential of calli derived from embryonic shoot of West Coast Tall cv. of coconut(Cocos nucifera L.)
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Date
2015-08
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Abstract
Maintenance of embryogenic potential of calli is important as the totipotency is often lost in a short time in vitro. This caters to the
need for year round availability of somatic embryos in a regenerable state. In the present study, 14 media combinations, with
either 2,4-D or picloram as auxin source, were tested for maintaining embryogenic calli obtained from embryonic shoot explants
of coconut. Irrespective of type and concentration of auxins, callusing was observed in all the media combinations. However, high
dose of 2,4-D (above 74.6 μM) in the initial medium resulted in intense browning and lesser percentage of callusing. Embryogenic
nature of calli could be maintained to a maximum of 21 weeks in medium supplemented with 2,4-D (74.6 μM) and subsequent
culturing into higher concentration of 2,4-D (90.4 μM). Gene expression studies carried out using qRT-PCR revealed that genes
such as ECP, GST, LEAFY and WUS were highly expressed in long term embryogenic calli (21 week old) and genes such as
SERK, GLP, WRKY and PKL in initial embryogenic calli (21 days old). The study concludes that coconut plumular calli could be
maintained for longer periods without compromising on the embryogenic potential of the calli.
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Keywords
Coconut, embryogenic calli, embryoic shoot, gene expression, histology, plumule
Citation
Journal of Plantation Crops,August 2015,Vol.43. No.2,p.105-116