A rapid methyl salicylate clearing technique for routine phase-contrast observations on female meiosis in Solanum
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Date
1987-05
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Abstract
A rapid clearing technique, involving methyl salicylate clearing and phase-contrast microscopy, that enables large-scale observations on meiosis and embryo sac development in intact ovules of potato, was developed. With high resolution phase-contrast optics the details observed in cleared ovules were as well defined as in microtome sections and 'abnormalities' associated with desynapsis and 2n-megaspore formation were readily detected. In potato this ovule clearing technique offers certain advantages over earlier clearing and staining-clearing techniques and permits bulk preparation of ethanol (70%) stored ovaries shortly (1-5-2 h) before observation.
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Solanum, clearing technique, methyl salicylate, phase-contrast microscopy, megasporogenesis, megagametogenesis
Citation
Journal of Microscopy Vol.146 Pt.2, May 1987,pp.157-162