A rapid methyl salicylate clearing technique for routine phase-contrast observations on female meiosis in Solanum

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dc.contributor.authorJongedijk, E.
dc.date.accessioned2014-09-12T09:16:50Z
dc.date.available2014-09-12T09:16:50Z
dc.date.issued1987-05
dc.description.abstractA rapid clearing technique, involving methyl salicylate clearing and phase-contrast microscopy, that enables large-scale observations on meiosis and embryo sac development in intact ovules of potato, was developed. With high resolution phase-contrast optics the details observed in cleared ovules were as well defined as in microtome sections and 'abnormalities' associated with desynapsis and 2n-megaspore formation were readily detected. In potato this ovule clearing technique offers certain advantages over earlier clearing and staining-clearing techniques and permits bulk preparation of ethanol (70%) stored ovaries shortly (1-5-2 h) before observation.en_US
dc.identifier.citationJournal of Microscopy Vol.146 Pt.2, May 1987,pp.157-162en_US
dc.identifier.urihttp://hdl.handle.net/123456789/5672
dc.language.isoenen_US
dc.subjectSolanumen_US
dc.subjectclearing techniqueen_US
dc.subjectmethyl salicylateen_US
dc.subjectphase-contrast microscopyen_US
dc.subjectmegasporogenesisen_US
dc.subjectmegagametogenesisen_US
dc.titleA rapid methyl salicylate clearing technique for routine phase-contrast observations on female meiosis in Solanumen_US
dc.typeArticleen_US

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