The spliced leader RNA gene array in phloem-restricted plant trypanosomatids (Phytomonas) partitions into two major groupings : epidemiological implications

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dc.contributor.authorDollet, M.
dc.contributor.authorSturm, N.R.
dc.contributor.authorCampbell, D.A.
dc.date.accessioned2014-05-19T09:41:24Z
dc.date.available2014-05-19T09:41:24Z
dc.date.issued2001
dc.description.abstractThe arbitrary genus Phytomonas includes a biologically diverse group of kinetoplastids that live in a wide variety of plant environments. To understand better the subdivisions within the phytomonads and the variability within groups, the exon, intron and non-transcribed spacer sequences of the spliced leader RNA gene were compared among isolates of the phloem- restricted members. A total of 29 isolates associated with disease in coconut, oil palm and red ginger (Alpinia purpurata, Zingibreaceae) were examined, all originating from plantations in South America and the Caribbean over a 12-year period. Analysis qf non-transcribed spacer sequences revealed 2 main groups, I and II ; group II could be further subdivided into 2 subgroups, IIa and IIb. Three classes of spliced leader (SL) RKA gene were seen, with SLI corresponding to group 1, SLIIa to group Ila, and SLIIb to group IIb. Two isolates showed some characteristics of both major groups. Group- specific oligonucleotide probes for hybridization studies were tested, and a multiplex amplification scheme was devised to allow direct differentiation between the 2 major groups of phloem-restricted Phytomonas. These results provide tools , for diagnostic and molecular epidemiology of plant trypanosomes that are pathogenic for commercially important flowers and palms.en_US
dc.identifier.citationParasitnlngy (2001), 122, 289-297en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1468
dc.language.isoenen_US
dc.subjectred gingeren_US
dc.subjectcoconuten_US
dc.subjectoil palmen_US
dc.subjectmini-exonen_US
dc.subjectPCRen_US
dc.subjecttandem arrayen_US
dc.titleThe spliced leader RNA gene array in phloem-restricted plant trypanosomatids (Phytomonas) partitions into two major groupings : epidemiological implicationsen_US
dc.typeArticleen_US

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