In Vitro Germination and Growth of Cacao Pollen
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2007-02
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This paper deals with pollen germination, pollen tube growth and pollen tube mitosis in cacao (Theobroma cacao L.). Good pollen germination and growth were obtained in a medium containing 15 % sucrose, 100 ppm boric acid, and 100 ppm calcium nitrate. Boron or calcium was necessary for proper pollen germination and tube growth, and both, if present, enhanced tube growth considerably. No pollen germination was observed at low temperatures (10°C), and maximum germination and tube growth were obtained at 35°C. Further increase in temperature to 40°C affected adversely both germination and tube growth. Pollen tube mitosis began within 2% hr of the initiation of pollen germination, and completed in 2-J hr. Pollen tube mitosis was normal in the cultivars studied.
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J.Plantn.Crops 1977 v-5 i-2 p-109-111