Mtha1, a Plasma Membrane H+-ATPase Gene from Medico truncatula, Shows Arbuscule-Specific Induced Expression in Mycorrhizal Tissue
| dc.contributor.author | Krajinski, F. | |
| dc.contributor.author | Hause, B. | |
| dc.contributor.author | Gianinazzi-Pearson, V. | |
| dc.contributor.author | Frankon, P. | |
| dc.date.accessioned | 2014-09-05T09:29:10Z | |
| dc.date.available | 2014-09-05T09:29:10Z | |
| dc.date.issued | 2002-12 | |
| dc.description.abstract | Transport processes between plant and fungal cells are key elements in arbuscular mycorrhiza (AM), where H+-ATP-ases are considered to be involved in active uptake of nutrients from the symbiotic interface. Genes encoding H+-ATPases were identified in the genome of Medicago truncatulo and three cDNA fragments of the l-T-ATPase gene family (Mthol-3) were obtained by RT-PCR using RNA from M. truncatula mycorrhizal roots as template. While Mtha2 and Mtha3 appeared to be con-stitutively expressed in roots and unaffected by AM development, transcripts of Mthal could only be detected in AM tissues and not in controls. Further analyses by RT-PCR revealed that Mtha~\ transcripts are not detectable in shoots and phosphate availability did not affect RNA accumulation of the gene. Localization of transcripts by in situ hybridization on AM tissues showed that /Wthol RNA accumulates only in cells containing fungal arbuscules. This is the first report of arbuscule-specific induced expression of a plant H+-ATPase gene in mycorrhizal tissues. | en_US |
| dc.identifier.citation | Plant Biol.4(2002) | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/5406 | |
| dc.language.iso | en | en_US |
| dc.subject | Arbuscular mycorrhiza | en_US |
| dc.subject | in situ hybridisation | en_US |
| dc.subject | plant nutrition | en_US |
| dc.subject | RNA accumulation | en_US |
| dc.subject | symbiosis | en_US |
| dc.title | Mtha1, a Plasma Membrane H+-ATPase Gene from Medico truncatula, Shows Arbuscule-Specific Induced Expression in Mycorrhizal Tissue | en_US |
| dc.type | Article | en_US |