Characterization of microsatellites from cacao–Moniliophthora perniciosa interaction expressed sequence tags

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dc.contributor.authorLima, L.S.
dc.contributor.authorGramacho, K.P.
dc.contributor.authorGesteira, A.S.
dc.contributor.authorLopes, U.V.
dc.contributor.authorGaiotto, F.A.
dc.contributor.authorZaidan, H.A.
dc.contributor.authorPires, J.L.
dc.contributor.authorCascardo, J.C.M.
dc.contributor.authorMicheli, F.
dc.date.accessioned2014-04-04T10:23:28Z
dc.date.available2014-04-04T10:23:28Z
dc.date.issued2008
dc.description.abstractTheobroma cacao L.–Moniliophthora perniciosa expressed sequence tags (ESTs) were converted into useful satellite markers for population analysis and genetic mapping. Forty-nine flanking primer pairs from TSH1188 (a resistant genotype) and Catongo (a susceptible genotype) ESTs were designed and screened for polymorphism analysis. Eleven were polymorphic, with an average of 3.81 alleles per locus and a total of 42 alleles. The satellite markers were tested on 21 cacao accessions and two bulked DNAs generated from 6 resistant and 6 susceptible plants from a segregating F2 (SCA6 9 ICS1) population for witches’ broom resistance. These results show that EST-derived microsatellites (short sequence repeats, SSRs) in Theobroma cacao have many potential applications in linkage mapping and the planning of crosses.en_US
dc.identifier.citationMol Breeding (2008) 22:315–318en_US
dc.identifier.urihttp://hdl.handle.net/123456789/241
dc.language.isoenen_US
dc.subjectcocoaen_US
dc.subjectESTsen_US
dc.subjectMicrosatelliteen_US
dc.titleCharacterization of microsatellites from cacao–Moniliophthora perniciosa interaction expressed sequence tagsen_US
dc.typeArticleen_US

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