Mayilvaganan, M.2014-06-242014-06-242007-02Plantation Crops Research and Development in the New Millennium,(Proceedings of PLACROSYM XIV)CPCRI, Kasaragod 2002 p-520-523http://hdl.handle.net/123456789/3249Separation of protein component from phytoplasma is important for its characterization and use as antigen for production of phytopiasma-specific antiserum, since proteins make the best antigens. Protein was isolated from purified phytoplasma of coconut root (wilt) disease by three methods and subjected to sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Comparative analysis of different methods showed that the method in which water-saturated phenol with 8-hydroxyquinoline and 0.1% sodium dodecyl sulphate were used, yielded more undegraded and readily soluble proteins that were distinctly separated in SDS-PAGE. The other two methods wherein water-saturated phenol with 8-hydroxyquinoline and liquid phenol alone was used, gave much lower protein yield that were insoluble and degraded in electrophoresis. SDS-PAGE analysis of the proteins fractionated by the three methods showed that 2 bands resolving at 28 and 29 kDa were observed only in the case of phytoplasma proteins isolated by water-saturated phenol, 8-hydroxyquinoline and 0.1% SDS method, which were observed as a single band in the other two methods. These results thus indicate that the water-saturated phenol, 8-hydroxyquinoline and 0.1% SDS method is most suitable for separation of protein from purified phvtoplasma.enArticle