Oulo N’NanValérie HocherJean-Luc VerdeilJean-Louis KonanKoffi BalloFanja MondeilBernard Malaurie2017-01-192017-01-192008CryoLetters 29(4), 339-350 (2008)http://hdl.handle.net/123456789/6843This study describes the use of an encapsulation-dehydration cryopreservation technique on coconut plumules (apical dome with three or four leaf primordia) excised from embryos. In order to establish a reliable cryopreservation process for plumules, several different key factors were tested: pretreatment duration, sugar concentration, dehydration period and freezing. In parallel, histological studies were performed to describe the structural changes of tissues and plumule cells subjected to dehydration and freezing. A good survival level of around 60% was obtained. However, after 8 months culture regrowth, this level decreased to a maximum of 20% which was achieved using sucrose treatment. In this paper we report for the first time the regeneration of leafy shoots from coconut plumules after cryopreservation.enCocos nucifera L.cryopreservationencapsulation-dehydrationhistologyplumulesucroseArticle