Michael R. LadischGeorge T. Tsao2014-09-202014-09-201978Journal of Chromatography, 166(1978) 85-100http://hdl.handle.net/123456789/5923The rapid separation of oligo- and monosaccharides on long, narrow columns packed with easily compressed, 4% cross-linked, cation-exchange resin adds a new dimension to the chromatography of carbohydrates on gel type supports: speedy analysis at low pressure. The discovery of a pressure-critical diameter effect in the packing of Aminex 50W-X4 (Ca2+) makes it possible to pack 60-cm long columns capable of separating malto- and cellodextrins in 12-15 min using water as the sole eluent. The surprising phenomena of a 50-fold increase in pressure due to an increase in column diameter from 6 to 8 mm is reported and reasons for this effect are explained. Equally noteworthy is the stability of the 6-mm columns. One column described in this report has been in continuous operation for over 2600 h. The application of low-pressure liquid chromatography to enzyme kinetics as well as to separation of oligo-and monosaccharides is also discussed.enArticle