HALINA KONONOWICZJANICK, J.2014-04-042014-04-041988FOLIA HISTOCHEMICA ET CYTOBIOLOGICA vol. 26 (1988) no. 4 pp.249-256http://hdl.handle.net/123456789/230Embryo formation from callus of Tft,eobroma cacao L. was associated with the changes in relationship between nuclear, nuCleolar and cell sizes and the content of basic proteins (FG-FCF~stained). Together with the increase in nuclear size of callus and proembryo cells the increase in the amount of nuclear basic proteins was found. Ib. the callus cells the increase in nucleolar protein content exceeded that in nucleolus size, whk:h led to the rise in basic protein concentration in the nucleolus. However, in the early stage of embryogenesis the increase in protein content was not so marked as that in callus, which indicated that embryogenesis involved a decrease in concentration of nucleolar basic proteins. Differences between callus and proembryo cells were also observed in the concentration of cytoplasmic proteins. The increase in size of callus celts was the same as the increasing amount of cytoplasmic proteins. In proembryos a significant increase in cell size was accompanied by only slight changes in cytoplasmic proteins. The stimulation of embryogenesis by 2.4-D resulted in an increase of nuclear concentration of basic proteins in proembryos. The intensification of embryogenesis involved the decrease of the concentration of nucleolar proteins together with the increase in concentration of basic cytoplasmic proteins.encocoanucleusnucleoluscell Sizesomatic EmbryogenesisArticle