Sreekrishna Bhat, S.Ratnambal, M.J.2014-06-172014-06-172007-02J.Plantn.Crops 1997 v-25 i-1 p-77-82http://hdl.handle.net/123456789/3016Germination and storage trials were carried out with pollen from West Coast Tall cultivar. The effect of various chemicals like sucrose, horic acid, potassium nitrate and calcium chloride upon in vitro germination of pollen was studied. The highest percentage of germination (68.8%) was achieved with a concentration of 10% sucrose and 10 ppm boric acid. Germination was not noticed in distilled water, tap water or in potassium nitrate solution. The pollen- tubes reached a length of 266um after 3 hours with a diameter of 42.6 urn. The viability of pollen was maintained when it was stored at 70 and 50% relative humidity (R.H.) for six days (with 45% germination) and eight days (with 56.8% germination) respectively. The pollen could be stored upto 12 days (with 53.4% germination) in a freezer compartment (at sub-zero temperature) for 90 days retaining a minimum - 50% pollen germination.enArticle