Aurora G. Del RosarioEmerita V. De Guzman2014-05-032014-05-032007-02-08The Philippine Journal of Science 1976 p-216-222http://hdl.handle.net/123456789/888Excised coconut makapuno embryos were sequentially cultured from liquid to solid medium using modified Whites basal medium and Murashige and Skoogs medium with varying levels of dextrose and other supplements. A low salt and high sugax concentration (Wo 8D) in the initial liquid medium and a high salt and moderate sugar concentration in the first transplant medium (Mo 4D) was found to be optimum for a balanced shoot and root development. During the liquid culture not all of the sugar supplied at S% was used up metabolically; a portion of it was active only as an osmotic agent. In the first transplant medium dextrose seems to be required solely as nutrient source.enArticle