Romila, P.P.Anitha KarunSajini, K.K.Jayadev, K.2014-06-302014-06-302004-12Journal of Plantation Crops 32(Suppl.) December 2004: 134-138http://hdl.handle.net/123456789/3511A protocol for in vitro active conservation of immature zygotic embryos was standardized and observed to be repeatable. Immature embryos collected from 10 and 11 months old nuts of two exotic germplasm viz., Cochin China Tall- (CCNT) and Malayan Yellow Dwarf- (MYD) cultivars of coconut were utilized for the study. Extracted embryos were stored (for 30 days) individually in 5 ml screw capped bottles containing 2 ml of storage medium such as sterile water alone (TI), sterile water with sucrose (7.5 mgfl) (T2), and !A strength of Y3 medium containing activated charcoal but without sucrose (T3). The embryos were retrieved on Y3 medium after one month of storage in different treatment media. Significantly higher percentage of germination and healthy plantlets could be obtained from T2 compared to other two treatments. Germination at 60 days after inoculation was significant with type and age factor. Regarding type, germination was more in Dwarf type (93.7%)whereas for CCNT it was :85.6%. Both shoot and root lengths were found to be significantly different among the three storage media compared.enCoconut (Cocos nucifera L.)germplasm collectionembryo culturestorage mediumArticle