Rajesh, M.K.Radha, E.Sajini, K.K.Anitha KarunParthasarathy, V.A.2014-07-162014-07-162005Journal of Plantation Crops, 2005, 33 ( 1 ) : 9-17http://hdl.handle.net/123456789/4153A procedure is outlined for regeneration of complete plantlets via organogenesis from plumular tissues of coconut. Callus was induced from plumular tissues in Y3 media supplemented with either 2,4-D (74.6 M) alone or 2,4-D (74.6 M) in combination with TDZ (4.54 M). The frequency of callus induction increased and the browning of explants was reduced when cytokinin (TDZ) was added along with the auxin (2.4-D) in the calus induction medium. The calli were subcultured at monthly intervals to media containing lower levels of 2,4-D and a constant level of either cytokinins (BA and TDZ) of polyamines (spermine and putrescine). Higher percentages of embryogenic calli, somatic embryoids and meristemoids were obtained in Y3 media supplemented with either sprernine of BA. Plantlets with balancedshoot and root formation were transferred to pots and established in the greenhouse. Histological studies of the differentiated tissues confirmed the development of shoot buds (organogenesis) and typical bipolar embryoids (somatic embryogenesis). Abbreviatipns: BA: 6-benzyladenine; 2.4-D: 2,4-dichlorophenoxyacetic acid; 1BA:indole-3butryic acid; TDZ : 1 - phenyl - 3(1,2,3-thiadiazol-5-yl) urea (Thidiazuron)enCoconutplant regenerationorganogenesissomatic embryogenesisArticle