Perera, P.I.P.Kularatene, J.D.J.S.Weerakoon, L.K.2014-04-262014-04-262011Cord-27(2) 2011 pp.26-37http://hdl.handle.net/123456789/654Experiments were conducted to compare the liquid medium with the media solidified with agar or phytagel.Selective subculturing and use of the embryo maturation medium supplemented with higher concentration of phytagel(0,5%; w/v) were also tested for reducing the vitrified embryos,Modified Eeuwens Y3 medium was used as the basal medium. By culturing the anthers on the medium solidified with phytagel (0.25%; w/v), direct embryo formation (86 .7%) and embryo conversion(21.5%) were significantly increased. Plant regeneration efficiency of anther derived embryos or calluses developed in the liquid culture medium was extremely low (2.4%) . Vitrification was further reduced by incorporating 0.5% (w/v) phytagel into the embryo maturation medium. Highest plant regeneration efficiency was obtained by exposing the embryos to 0.5%(w/v) phytagel for 21 days,which reduced vitrification by 42%. Furthermore, selective subculturing of the embryos was effective for reducing vitrification.enAnther cultureCocos nuciferaPhytagelhistologydoubled haploidArticle