Erlinda P. RilloBelen F. Paloma, M.2014-05-192014-05-192007-02-08Plant Genetic Resources News Letter v.86 p-1-4http://hdl.handle.net/123456789/1475Embryo culture of coconuts can circumvent problems of storage and transport caused by the weight and bulk of the nuts and the absence of dormancy. This paper describes a technique that can be routinely used for collecting large numbers of embryos. The embryos were extracted in the field, brought to a simple isolation room, washed in ethyl alcohol, disinfected in 1OO% commercial bleach for 20 minutes and then rinsed again. Sterilized embryos were sealed in sterile plastic bags and then transported in cold storage. Redisinfection in 100% commercial bleach for 5 minutes prior to culture under aseptic conditions was necessary to reduce contamination rates. This procedure did not adversely affect embryo germination.encoconutzygotic embryo-storagezygotic embryo-transportin vitro cultureArticle