Jongedijk, E.2014-09-122014-09-121987-05Journal of Microscopy Vol.146 Pt.2, May 1987,pp.157-162http://hdl.handle.net/123456789/5672A rapid clearing technique, involving methyl salicylate clearing and phase-contrast microscopy, that enables large-scale observations on meiosis and embryo sac development in intact ovules of potato, was developed. With high resolution phase-contrast optics the details observed in cleared ovules were as well defined as in microtome sections and 'abnormalities' associated with desynapsis and 2n-megaspore formation were readily detected. In potato this ovule clearing technique offers certain advantages over earlier clearing and staining-clearing techniques and permits bulk preparation of ethanol (70%) stored ovaries shortly (1-5-2 h) before observation.enSolanumclearing techniquemethyl salicylatephase-contrast microscopymegasporogenesismegagametogenesisArticle