Iyer, R.D.2014-06-042014-06-042007-02-08In : Advances in Coconut Research and Development 1993 (Eds.)MK Nair, HH Khan; P Gopalasundaram ; EVV Bhaskara Rao, Oxford & IBH Publishing Co. Pvt. New Delhi, 759 p(International symposium on Coconut Research and Development (ISOCRAD*II), 26-29 November 1991, CPCRI, Kasaragod) p.149-159http://hdl.handle.net/123456789/2355The first report of successful induction of somatic embryoids and their subsequent organisation into clonal plantlets was from CPCRI in 1984. Using tender leaf segments from one to two-year old WCT seedlings, somatic embryoids were induced in 16 weeks on a modified Y-3 medium supplemented with high levels of auxin (NAA), inositol and casein hydroiysate. After a process of hardening, four of these plantlets have been successfully field planted in January 1989 and are growing well. More recently, with a view to reproducing the above success with mature palm tissues, a non-destructive method of tissue extraction has been standardised. Rachilla explants have so far given only shoot-like outgrowths, but no true leaves yet, whereas anther culture gave only multi-celled pollen embryoids.encoconut tissue culturetissue culturebiotechnologyArticle