Krajinski, F.Hause, B.Gianinazzi-Pearson, V.Frankon, P.2014-09-052014-09-052002-12Plant Biol.4(2002)http://hdl.handle.net/123456789/5406Transport processes between plant and fungal cells are key elements in arbuscular mycorrhiza (AM), where H+-ATP-ases are considered to be involved in active uptake of nutrients from the symbiotic interface. Genes encoding H+-ATPases were identified in the genome of Medicago truncatulo and three cDNA fragments of the l-T-ATPase gene family (Mthol-3) were obtained by RT-PCR using RNA from M. truncatula mycorrhizal roots as template. While Mtha2 and Mtha3 appeared to be con-stitutively expressed in roots and unaffected by AM development, transcripts of Mthal could only be detected in AM tissues and not in controls. Further analyses by RT-PCR revealed that Mtha~\ transcripts are not detectable in shoots and phosphate availability did not affect RNA accumulation of the gene. Localization of transcripts by in situ hybridization on AM tissues showed that /Wthol RNA accumulates only in cells containing fungal arbuscules. This is the first report of arbuscule-specific induced expression of a plant H+-ATPase gene in mycorrhizal tissues.enArbuscular mycorrhizain situ hybridisationplant nutritionRNA accumulationsymbiosisArticle